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CN106046123A 一种鸡传染性法氏囊病病毒VP2蛋白及其制备方法与应用及试剂盒 无效 - 驳回

一种鸡传染性法氏囊病病毒VP2蛋白及其制备方法与应用及试剂盒
摘要：
本发明提供一种鸡传染性法氏囊病病毒VP2蛋白及其制备方法与应用，具体地，编码该VP2蛋白的核苷酸序列含有如SEQ ID No.1所示序列。制备该VP2蛋白方法包括以下步骤：1)化学合成SEQ ID No.1序列，2)构建表达载体和3)转染sf9细胞。该VP2蛋白可用于制备鸡传染性法氏囊病的疫苗。该SEQ ID No.1是根据昆虫细胞偏爱密码子对鸡IBDV VP2基因进行密码子优化而得，比一般的从IBDV病毒扩增的VP2基因，更适应在昆虫细胞中表达，更能提高蛋白表达量。
摘要（英）：

The invention provides chicken infectious bursal disease virus VP2 protein and a preparation method and application thereof and a kit. Particularly, a nucleotide sequence for coding the VP2 protein contains the sequence as shown in SEQ ID No.1. The method for preparing the VP2 protein comprises the following steps of chemically synthesizing the SEQ ID No.1 sequence, constructing an expression vector and transfecting sf9 cells. The VP2 protein can be used for preparing a vaccine for a chicken infectious bursal disease. The SEQ ID No.1 is obtained by conducting codon optimization on a chicken IBDV VP2 gene according to insect cell preference codon, and therefore compared with a general VP2 gene which is amplified from an IBDV virus, the SEQ ID No.1 is more suitable for being expressed in insect cells and can increase the protein expression quantity.

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