The invention relates to a heavy metal detection sensing material, and a preparation method and an application thereof. The method comprises the following steps: a monomer and ethyl 2-bromoisobutyrate are added into butane; diisopropylethylamine is added; copper N,N-di-n-butyl dithiocarbamate is added; the mixture is cooled to room temperature; bis(2,6-difluoro-3-(1H-pyrrol-1-yl)phenyl)titanocene and ethanol are added; under stirring, a light reaction is carried out for 8h; when the reaction is finished, reaction liquid is added into water; an upper-level solution which is an acrylic acid prepolymer solution is taken; octamethylcyclotetrasiloxane and a rhodamine compound are added into the acrylic acid prepolymer solution; phthalic anhydride is added; the solid content of the solution is regulated to 15-20%, such that a detection material solution is obtained; a polyester composition is added into an extruder, and a substrate sheet material is obtained through extrusion; the surface of the substrate sheet material is coated with the detection material solution; and hot press treatment is carried out, such that the heavy metal detection sensing material is obtained. The material has excellent mechanical performance, and satisfies the development and application of the heavy metal detection sensing material.