The invention provides a purifying method of adipose-derived stem cells. The method comprises the following steps: primary adipose-derived stem cell culture and CD90+ cell population purification. The invention further provides application of the purified adipose-derived stem cells in the aspects of osteogenic induction and chondrogenesis. Obtained CD90+ cell population in ADSCs has high stem cell differentiative potential, using efficiency and repairing effect of ADSCs in tissue engineering can be improved, and under the same induction condition, osteogenesis and chondrogenesis are improved by 3-5 times and 5-7 times respectively.