利索-全球专利检索

  1. 登录
  2. 注册
发明授权
CN106047911B 构建的鲤凋亡基因、载体及其应用 有权

构建的鲤凋亡基因、载体及其应用
摘要：
本发明涉及一种鲤凋亡基因、载体及其应用。所构建的鲤凋亡基因，从5’至3’依次由鲤MT‑1启动子、鲤Caspase‑8小亚基(P10)编码序列、鲤Caspase‑8大亚基(P20)编码序列、鲤MT‑1polyA信号序列组成。本发明的所述鲤DNA疫苗载体，包括真核表达载体及插入在所述真核表达载体中的上述鲤基因的凋亡基因。本发明以鲤的MT启动子和Caspase‑8为基础，构建诱导型凋亡基因，将其插入pEGFP‑N1载体构建“自杀性”DNA疫苗载体，该凋亡基因可以响应金属离子(例如Zn2+)等条件的诱导，表达Caspase‑8诱导转染细胞的凋亡，可应用于鲤DNA疫苗的制备，以进一步提高DNA疫苗的安全性。
摘要（英）：

The invention relates to a constructed carp apoptotic gene, a vector and application of the gene. From 5' to 3', the constructed carp apoptotic gene is sequentially composed of carp MT-1 promoter, caspase-8 small subunit (P10) coding sequence, caspase-8 large subunit (P20) coding sequence, and carp MT-1polyA signal sequence. The carp DNA vaccine vector of the invention includes a eukaryotic expression vector and an apoptotic gene of the above carp gene in the eukaryotic expression vector. The inductive apoptotic gene is constructed based on carp MT promoter and caspase-8, and after being inserted to pEGFP-N1 vector to construct 'suicide' DNA vaccine vector, the apoptotic gene may respond to the induction of the conditions such as metal ions (e.g., Zn2+) to express apoptosis of caspase-8 induced transfection cells and may apply to the preparation of carp DNA vaccines to further improve safety of DNA vaccines.

公开/授权文献
0/0