The invention discloses a production method of PS plate interleaving paper. The production method comprises the process steps as follows: respective beating of softwood pulp and hardwood pulp, pulp blending and addition of auxiliary materials, wet paper sheet making, pre-drying, surface sizing, after-drying, calendaring and finishing. Through research of pulp selection, pulp proportioning, a beating process and addition of functional chemical assistants to the pulp, adoption of film transfer surface sizing treatment as well as integration of finishing measures such as a soft calendaring equipment and the like, production of qualified PS plate interleaving paper is executed according to related paper machine parameters, and the method has great significance in optimization of product structure for a company and improvement of profitability of the company.