The invention relates to a method for detecting content and molecular weight of epsilon-polylysine in beverage. According to characteristics of epsilon-polylysine polycations, adsorption extraction is performed on the beverage by using macroporous weakly acidic cations D152, and then elution is performed by using hydrochloric acid of 1 mol/L, so that the epsilon-polylysine of 95% in the beverage can be extracted and obtained, and interference caused by other matters in the beverage to subsequent detection is avoided; by detection of the ultraviolet absorption strength of the extracted epsilon-polylysine under 215 nm, the content of the epsilon-polylysine in a sample is calculated and obtained; by detection of the retention time of the epsilon-polylysine in a gel filtration chromatographic column, the molecular weight of the epsilon-polylysine in the sample is calculated and obtained. By adoption of the method for detecting the content and the molecular weight of the epsilon-polylysine in the beverage, the epsilon-polylysine contained in the beverage can be highly efficiently extracted, and the content and the molecular weight of the epsilon-polylysine in the beverage can be detected at the same time.