The invention provides an identification board for identifying seed storage positions in a refrigerator and a manufacturing method of the identification board. The identification board comprises a base and clamping grooves, wherein the base is made from a stainless steel plate and is of a rectangular structure; and the clamping grooves are such formed that the upper parts are kept straight, two ends are fixed on the sidewalls of each seed storage shelf by virtue of screws and three faces of the edges of middle lower parts are coiled inwards. Each inserting board is made from a PVC (polyvinyl chloride) board and is of a rectangular structure; and each inserting board includes contents of shelf number, container type and storage position number, and the contents are ink-jet printed. The identification board disclosed by the invention has the advantages of being simple in designed structure, excellent in low-temperature resistance and moisture resistance, long in service life, clear to identify, easy to update and the like; the seed storage positions, in correspondence to the different storage shelves in the refrigerator, can be effectively displayed, so that seed management personnel can rapidly find out required seeds or seed storage positions from the refrigerator which is at minus 18-20 DEG C; therefore, seed management efficiency is improved and the life security of the management personnel is guaranteed.