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发明公开
CN106069792A 一种三七紫色素愈伤组织的诱导方法 有权

一种三七紫色素愈伤组织的诱导方法
摘要：
本发明公开了一种三七紫色素愈伤组织的诱导方法，以三七植株幼嫩的茎、叶、花或花药作为外植体，经过灭菌、接种、及诱导培养，得到三七紫色素愈伤组织；所述诱导培养的光照控制为4～8小时光照、4～8小时黑暗，交替进行。其诱导培养的培养基为MS+0.1～2mg/L 2,4‑D+0.1～2mg/L BA+0.002～1mg/L TDZ+30～60g/L蔗糖；或MS+0.1～2mg/L NAA+0.1～2mg/L BA+0.002～1mg/L TDZ+30～60g/L蔗糖。本发明通过对光照时间、培养基、湿度、温度等条件控制，培养出富含紫色素、诱导率高的愈伤组织。本发明通过缩短光照与黑暗的交替时间的方法有效的提高了三七紫色素的含量，并在培养基中加入TDZ及与其他植物激素的搭配，使三七愈伤组织的诱导率明显提高，并有利于紫色素的形成。本发明为大量提取三七愈伤组织中的紫色素奠定了基础。
摘要（英）：

The invention discloses a method for induction of a panax notoginseng purpurin callus. Tender stems, leaves, flowers or anthers of panax notoginseng plants are used as explant, and sterilization, inoculation and induction culture are conducted on the explant to obtain the panax notoginseng purpurin callus; 4-8 h of illumination and 4-8 h of dark treatment are conducted alternately for induction culture; a culture medium for induction culture is MS+0.1-2 mg/L 2,4-D+0.1-2 mg/L BA+0.002-1 mg/L TDZ+30-60 g/L saccharose or MS+0.1-2 mg/L NAA+0.1-2 mg/L BA+0.002-1 mg/L TDZ+30-60 g/L saccharose. By controlling illumination time, the culture medium, moisture, temperature and other conditions, the callus rich in purpurin and high in inductivity is cultured. By shortening the alternation time of illumination and dark treatment, the content of panax notoginseng purpurin is effectively increased; by adding TDZ to the culture medium to be matched with other plant hormones, the inductivity of the panax notoginseng callus is improved remarkably, and formation of purpurin can be achieved easily. The method lays a foundation for mass extraction of purpurin in the panax notoginseng callus.

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