The invention discloses a method for extracting coronatine from fermentation liquid and refining coronatine. Coronatine is obtained by adsorbing the fermentation liquid containing coronatine with an adsorbent. Experiments prove that the adsorbent adopted in the method is good in stability, easy to regenerate, high in coronatine adsorption rate and adsorption speed and short in production period; the method has the advantages of being simple in process, very easy and convenient to operate, mild in condition, free of destructive effects on coronatine, capable of achieving solvent recycling, low in cost and suitable for large-scale industrial production.