The invention discloses a mink pseudorabies inactivated vaccine and a preparation method and a purpose thereof. The mink pseudorabies inactivated vaccine comprises an inactivated pseudorabies virus strain PRV/DL14/08 with collection number of CGMCC No.12340. The invention also discloses the preparation method of the mink pseudorabies inactivated vaccine. After proofing by experiment, the prepared mink pseudorabies inactivated vaccine has the advantages that the safety is good; after the mink is performed with immunization, the ideal immunization protection effect can be produced, so that the mink pseudorabies inactivated vaccine can be used a candidate vaccine strain for preventing and controlling the pseudorabies of mustelidae animals, such as mink.