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发明授权
CN106086167B 一种快速鉴定大黄鱼、小黄鱼及棘头梅童鱼的引物序列及方法 失效 - 权利终止

一种快速鉴定大黄鱼、小黄鱼及棘头梅童鱼的引物序列及方法
摘要：
本发明涉及一种快速鉴定大黄鱼、小黄鱼及棘头梅童鱼的引物序列及方法。引物序列为：1F:5’‑GGAAAGAGCCAGGAAAGC‑3’；1R:5’‑GGCGGAACTCTGAGCAAA‑3’。方法包括：(1)个体基因组DNA的提取；(2)序列扩增的特异性引物合成与PCR扩增；(3)取PCR产物经琼脂糖凝胶电泳检测、拍照及记录，根据扩增产物的不同迁移距离获得3种鱼各自的特异性DNA片段图谱，参照附图进行判别。本发明能简单、准确区分出3种鱼的个体样品，为外形相近3种经济鱼类的种质鉴定提供一种新型快速研究方法。
摘要（英）：

The invention relates to a primer sequence and a method for rapid identification of pseudosciaena crocea, larimichthys polyactis and collichthys lucidus. The primer sequence is as follows: 1F:5'-GGAAAGAGCCAGGAAAGC-3' and 1R:5'-GGCGGAACTCTGAGCAAA-3'. The method includes the steps of (1) extracting individual genomic DNA; (2) synthesizing a specific primer for sequence amplification and performing PCR (Polymerase Chain Reaction) amplification; and (3) detecting the PCR product through agarose gel electrophoresis, taking pictures and performing recording, obtaining respective specific DNA fragment spectrograms of the three kinds of fishes according to different migration distance of the amplification products, and performing distinguishing in accordance to attached spectrograms. The method can simply and accurately distinguish individual samples of the three kinds of fishes, and provides a new fast research method for germplasm identification of the three kinds of economic fishes with similar appearance.

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