The invention relates to the technical field of connectors for gas sampling and detector sample introduction, in particular to a single-closing and opening rapid connector. The single-closing and opening rapid connector comprises a moving end and a fixed end, wherein the moving end comprises an upper connector, a lower connector, a connection disc, a rotation ball, an upper connection pipe and a lower connection pipe; the lower connector is provided with a lower ball base; the lower connection pipe is located on the bottom of the lower ball base and penetrates through the lower connector; the connection disc is fixed on the bottom of the lower connector; an upper ball base is arranged in the upper connector; an opening groove used for rotating the upper connection pipe is formed in one side of the upper connector; the upper connector is provided with inner threads matched with outer threads; the upper connector is rotatably fixed on the lower connector; the upper connection pipe extends out of the center of the opening groove; and the fixed end comprises a connection pipe, so that the upper connection pipe is inserted in in a sealed manner. The rapid connector is high in reliability and simple and convenient for use, and is especially suitable for large-scale sample acquisition and analysis.