The invention discloses a positioning and connection device for a liquid crystal screen and protective glass. The device comprises a positioning block used to position the protective glass and the liquid crystal screen; the positioning block is in a fitted cup-joint connection with a dismounting block; and a dismounting jacking pillar which can be relatively used to push out the protective glass and the liquid crystal screen placed on the positioning block is disposed on the dismounting block. The positioning and connection device for the liquid crystal screen and the protective glass has the advantages that the structure is simple and practical; use is rapid and convenient; the problem of accurate positioning of the liquid crystal screen and the protective glass can be solved; the liquid crystal screen can be dismounted rapidly after adhesion of the protective glass; a production percent of pass can be increased; production efficiency is greatly increased; and the device has broad application prospect.