The invention relates to a method for producing a mushroom in-situ specimen. The method comprises the following steps of: selecting materials with uniform mushroom sticks, complete and unbroken mushroom sticks, basically consistent size of fruiting bodies on the mushroom sticks, uncracked mushroom caps, no factors influencing view such as incomplete mushrooms, partial mushrooms and mildew points as well as no plant diseases and insect pests or mechanical damage for producing a specimen; prefreezing the mushroom sticks to below -40 DEG C, putting into a freeze drying oven, and drying; setting temperature of a cold trap to be lower than -50 DEG C, and in a sublimation drying stage, setting temperature to be ranged from -20 DEG C to -18 DEG C and pressure to be 60-80Pa; and in a desorption drying stage, setting temperature to be 40-45 DEG C and pressure to be 30-50Pa; and after drying is finished, putting the mushroom sticks into a transparent container with the bottom paved with a drying agent layer, and finally sealing the container, so that the mushroom in-situ specimen with an undamaged condition is obtained.