The invention provides a trolley of a freezer and a manufacturing method of the trolley. The trolley of the freezer consists of an inwards concave double-layer table surface, two handles and universal wheels, wherein the frame of the double-layer table surface is formed by the welding of stainless steel rectangular pipes, the table surfaces are manufactured by stainless steel plates, and a high-density plate and groove-shaped reinforcing ribs are used for strengthening the supporting intensity of the bottom of the double-layer table surface; four rectangular steel pipes between two table surfaces are welded to four corners of each of the two table surfaces; the two handles are positioned on the front end and the rear end of the outer frame of an upper-layer table plate, and each of the handles is formed by the welding of the stainless steel rectangular pipes and circular pipes; and the universal wheels are positioned on the four corners of the bottom surface of a lower-layer table plate, and are iron core universal wheels. According to the trolley disclosed by the invention, massive seed storage pots can be fast transported from a drying room to the freezer through the trolley, and the trolley freely passes through narrow gangways of the freezer and arrives at the specific position of the storage pots, so that the working efficiency is effectively improved, and the life safety of a seed administrator is guaranteed.