The invention provides a high-quality rust-proof antibacterial coating and preparation and application methods thereof. The high-quality rust-proof antibacterial coating is formed by combining a first component and a second component, the first component comprises aryl acrylamide, dimethylbenzene, isopropanol, kieselguhr and coal tar asphalt, and the second component comprises polyester resin, methyl ethyl ketone, dimethylbenzene, titanium dioxide, carbon black, zinc powder, carvacrol, china clay powder and aluminum paste. According to the preparation method of the high-quality rust-proof antibacterial coating, the process is simple and easy to carry out, production efficiency is high, the application cost is low, operation is easy, implementation is easy, the produced product is stable in quality, and high popularization and application value is achieved.