The invention relates to an iteration identification and suppression method of seismic channels containing single frequency interference. The method mainly solves the problems that in the prior art, the frequency of single frequency interference cannot be estimated precisely, and whether a seismic channel contains single frequency interference cannot be identified and processed precisely. The method can automatically identify whether seismic channels to be processed contains single frequency interference or not one by one without the participation of people, moreover, the method is not limited by the seismic channel processing domain, the amplitude, phase position, and frequency information of single frequency interference can be estimated precisely, and thus the single frequency interference is effectively suppressed.