The invention provides a method and a system for tracing multi-level association paths of two enterprises. The method comprises the steps of in response to search query, placing a to-be-inquired enterprise in a first database and placing a target enterprise in a second data base; according to information types, establishing enterprise nodes and staff nodes, and establishing the level association relations between the enterprises; collecting enterprise information on the internet, and updating the enterprise node and staff node data according to the level association relations; in response to the search query, inquiring association path sets of nodes in the first database and information in the second data base in a definite relation path depth successively, and circularly adding newly-found association path elements and node elements to a third database until the query is over; outputting storage results in the database and determining the association relation information between the to-be-inquired enterprise and the target enterprise.