The invention relates to a coating for preventing intermetallic bonding and particularly relates to a water-based steel-titanium bonding prevention coating and an application thereof in a titanium ingot preparation process. The water-based steel-titanium bonding prevention coating is prepared from the following components in parts by weight: 50-150 parts of water-based film forming agent, 0-50 parts of Cu powder, 400-450 parts of Al2O3 powder, 300-350 parts of talc powder and 0-50 parts of Fe2O3 powder. According to the invention, the coating can ensure that a titanium pipe does not experience a bonding reaction with a roller or a winch of steel equipment in a rolling or perforation process at 900 DEG C, and the yield and the production efficiency of titanium material processing are improved; and moreover, the technology is simple and easy to operate, the coating is environment-friendly and pollution-free, and the preparation process is simple.