The invention belongs to the technical field of oil production engineering of oilfields, particularly relates to a water injection well layered testing and regulation process device and a testing and regulation method thereof, and solves the problem that overall process cost is over high in a cable directly controlled water distribution method. According to the water injection well layered testing and regulation process device and the testing and regulation method thereof, a ground measurement and control system and a layered water injection tubular column with an intelligent water distributor (7) are used, pressure-flow curves of various layers are measured, and layer water injection amount is realized by current opening of a wellhead water injection valve (2) with injection pressure and flow. The water injection well layered testing and regulation process device and the testing and regulation method thereof have the advantages that the over high cost in the cable directly controlled water distribution method is reduced; testing and regulation can be realized on the ground; and a testing and regulation instrument is not required to trip in the well and the like.