The invention discloses an acoustic surface wave sensor manufacturing method based on the nano imprinting technology. The method comprises steps that a primary imprinting template on which a distribution pattern of an interdigital transducer and reflecting gratings at left and right sides of the interdigital transducer are carved is generated; the primary imprinting template is utilized to carry out continuous printing on a substrate through a step-by-step repetition imprinting method, the substrate is made to become a secondary imprinting template having multiple patterns identical to the pattern on the primary imprinting template; and the secondary imprinting template is utilized to imprint an acoustic surface wave sensor. The method is advantaged in that generation of the interdigital transducer can be accurately controlled, quite high measurement precision and consistency can be realized, insertion loss of a high-frequency acoustic surface wave device can be reduced through reducing interdigital resistance of the interdigital transducer, a sensorquality factor Q value is stably improved, quite high practicality is realized, manufacturing cost of the interdigital transducer can be reduced, and no pollution is realized.