The invention belongs to the technical field of bio-detection and particularly discloses a kit of high-effectively and quantitatively detecting Golgi apparatus 73 (GP73). In the invention, for the first time, an anti-GP73 polyclonal antibody and an anti-GP73 monoclonal antibody are mixed to obtain a combined capture antibody. The technology, instead of a conventional method in which only is one of the anti-GP73 polyclonal antibody or the anti-GP73 monoclonal antibody employed as a capture antibody, can significantly improve specificity and sensitivity of immune-detection and achieve a lower detection limit since the two antibodies hereinabove are mixed. The kits in the invention have strong specificity and sensitivity, lower detection limit and good linearity conditions.