The invention discloses a method for analyzing the immunological difference between two states of individuals. The method comprises the following steps: acquiring first sequencing data and second sequencing data; splicing a first read segment in the first sequencing data and a second read segment in the second sequencing data to obtain a first spliced sequence and a second spliced sequence; comparing the first spliced sequence and the second spliced sequence with various CDR3 reference sequences to obtain a first CDR3 sequence and a second CDR3 sequence; comparing the difference between a first high-frequency CDR3 sequence proportion and a second high-frequency CDR3 sequence proportion, and determining the distinguishing effect of the high-frequency CDR3 sequence proportions with statistical significance on the first state and the second state. The invention further discloses a method and/or a device for assisting in determining the states of the individuals.