The invention discloses a spent fuel pool which comprises a group of pools. Separating devices are arranged between the adjacent pools. The separating devices each comprise a bridge frame, a sluice gate and a sealing locking device. The invention discloses the spent fuel pool and a using method thereof. The spent fuel pool can be quickly started or shut down and effectively transport fuel rods, is small in pollution and good in sealing performance. A sealing device has simplicity in replacement and is long in service life.