The invention discloses a self-walking greenhouse air curtain type electrostatic spraying vehicle. The self-walking greenhouse air curtain type electrostatic spraying vehicle comprises a vehicle frame on wheels, a support frame, a guide rail, an adjustable pesticide supply system, an adjustable air supply system, a lithium battery, a differential driver, a walking motor, a DC-DC voltage-reduction power supply module, a high-voltage electrostatic generator, an electrostatic spray head, a supervisory computer, an electromagnetic valve, a high-resolution camera and a long-range mobile phone terminal, wherein the vehicle frame is fixed to the support frame; the lithium battery, the differential driver and the walking motor are fixed on a bottom plate of the spraying vehicle; the guide rail, the DC-DC voltage-reduction power supply module and the high-voltage electrostatic generator are fixed on the support frame; the adjustable pesticide supply system and the adjustable air supply system are mounted on the guide rail; and the high-resolution camera is located at the top of the spraying vehicle, and the high-resolution camera, the supervisory computer and the electromagnetic valve are sequentially connected so as to control pesticide applying quantity. According to the self-walking greenhouse air curtain type electrostatic spraying vehicle disclosed by the invention, a long-range control technique, an image processing technique and an air supply electrostatic pesticide application technique are combined, so that the charging effect is good, and the electrostatic adsorption capacity is high; and compared with a conventional air supply manner, the self-walking greenhouse air curtain type electrostatic spraying vehicle has the advantages of being good in sprinkling quality and uniform in distribution of liquid spraying quantity. Under the premise that the utilization rate of pesticides and killing effects are guaranteed, operations of image collection, affirmation of insect pest grades, autonomic walking, and control on pesticide application are all automatically completed, so that the labor capacity is reduced, and the efficiency is improved.