The invention provides an analysis method of eye pattern jitter. The analysis method comprises: acquiring a string of continuous signals without an acquisition blank space in the middle and generating an eye pattern corresponding to the continuous signals; determining a datum point of the eye pattern; generating a histogram by data in a calculation range of the eye pattern; carrying out normalization processing on the data of the histogram; traversing from left and right ends of the normalized histogram respectively, and determining a left tail part and a right tail part of the normalized histogram respectively; obtaining an initial variance and an initial mean value of Gaussian distribution, which correspond to the left tail part and the right tail part respectively; acquiring the fitting degree between the left tail part and the right tail part of the normalized histogram, and an actual left tail part and an actual right tail part of the continuous signals respectively, and taking the mean value and the variance, which correspond to the minimum value of the fitting degree, as an optimal mean value and an optimal variance of the left tail part and the right tail part of the normalized histogram respectively; generating actual mean values of the left tail part and the right tail part and actual variances of the left tail part and the right tail part; determining random jitter; or determining a peak-to-peak value of the jitter.