The invention relates to a calibration method and discloses a high energy beam spot calibration method which comprises the following steps: a beam spot is adjusted to preset coordinates, the beam spot is enabled to be in a preset state, circularity of the beam spot is recorded while the beam spot is in the preset state, a beam spot focusing value is changed at least once while beam spot astigmatism remains unchanged, and a function relationship between beam spot position parameters and the focusing value is generated. According to the high energy beam spot calibration method, the function relationship between the beam spot position parameters and the focusing value is generated, the position of the beam sport can be calibrated according to the function relationship and the circularity of the beam spot in the preset state, effects exerted on the position of the beam spot by focusing can be calibrated, beam spot calibration can be prevented from effects of image distortion, and a complex position relationship between an imaging device and a plane where the beam spot is positioned does not need to be solved.