The present invention provides a transmission line anti-freeze strain clamp and a manufacture method thereof. The transmission line anti-freeze strain clamp comprises an aluminum tube, a wire, a steel anchor, a rubber injection hole, and vulcanized rubber. The aluminum tube has a cavity. The wire stretches into the cavity of the aluminum tube from a first end of the aluminum tube. The steel anchor stretches into the cavity of the aluminum tube from a second end of the aluminum tube. The wire is connected with the steel anchor in the aluminum tube. The rubber injection hole is disposed on the aluminum tube and is communicated with the cavity of the aluminum tube. The vulcanized rubber enters the cavity of the aluminum tube through the rubber injection hole. The manufacture method comprises the step of injecting liquid vulcanized rubber into the strain clamp from the rubber injection hole of the aluminum tube till the vulcanized rubber overflows a junction of the aluminum tube and the steel anchor on the second end of the aluminum tube. When water is accumulated in the aluminum tube cavity and the existing strain clamp bears a larger tension, the accumulated water freezes at low temperature so as to cause deformation or break of the aluminum tube. The transmission line anti-freeze strain clamp solves the problems in the prior art.