The invention discloses a remote switching operation method based on comprehensive visualization and anti-error topology analysis, and belongs to the field of transformer substation dispatching control. Through the technical scheme, comprehensively visual online monitoring of an isolating switch is combined with a security protection technology based on four-layer anti-error topology, so that the security and reliability of remote operation projects of the isolating switch at a dispatching end are guaranteed. The method consists of two aspects, namely a visual operation mode and four-layer anti-error security assurance. Through the visual operation mode, reliable information assurance is supplied for the operation of the isolating switch, and the state of the isolating switch can be effectively monitored under an unattended condition; and through four-layer anti-error locking, the reliability of the operation is guaranteed, switching misoperation can be effectively avoided, and the security of the operation is guaranteed.