The invention belongs to the field of biotechnology and discloses an egg yolk antibody mouth wash capable of preventing and treating ozostomia and a preparation method of the egg yolk antibody mouth wash. The mouth wash comprises the following components in parts by weight: 1-10 parts of specific complex egg yok antibody preparation, 1-10 parts of a Chinese herb extract, and 100 parts of sterile water. The specific complex egg yolk antibody preparation is a complex antigen prepared from porphyromonas gingivalis, fusobacterium nucleatum and a bacterium internationally named Solobacterium moorei, a laying hen is primarily immunized and then immunized in a booster manner for five times all together, an egg is collected from the first booster immunization, an egg yolk liquid is obtained, separated and purified to obtain a specific complex egg yolk antibody. The egg yolk antibody mouth wash contains both the specific complex egg yolk antibody and an important extract, the breeding of microorganisms in an oral cavity is effectively inhibited, the ozostomia is prevented and treated, the normal flora of the oral cavity is not affected, and the mouth wash is safe and harmless.