The invention discloses a new durian fermented beverage preparation method which is as follows: juicing fresh durian, hydrolyzing with pectinase, centrifuging, homogenizing and sterilizing, and using dry active yeast for primary fermentation; mixing primary fermentation liquor with fresh non-antibiotic cow milk and white granulated sugar, homogenizing and sterilizing, and using a probiotic lactic bacteria compound starter culture for secondary fermentation; and after aseptic canning and after-ripening process, finally preparing a new durian fermented beverage. The new durian fermented beverage prepared by the method has fine and smooth texture, uniform colour and lustre and unique taste; the living bacterium number is greater than 108 cfu/mL, the acidity can be up to 48.7 + /-2.2-degree T, the viscosity can be 15.86 + /-3.1 cp, the soluble solid content can be (10.05 +/-1.58) %, the protein content is greater than or equal to 0.88%, and the alcohol content is less than or equal to 1%. In addition, the method effectively solves the problem of higher durian preservation transportation cost, and can improve the durian itself ''undesirable smell'' without addition of any additives, and the new durian fermented beverage is good for more consumers to accept, and is a new fermented beverage product.