The invention relates to the field of articles of daily use, in particular to a manufacturing method of a garbage bag with beneficial bacteria and the garbage bag. The manufacturing method comprises the steps that 1, sticky matter free of germicidal ingredients and liable to absorb water is added into a beneficial bacterium base material for mixing, and sticky paint is obtained after blending is carried out, specifically, the beneficial bacterium base material is a beneficial bacterium cultivation material with a beneficial bacterium group; and 2, the inner wall of the garbage bag is coated with the sticky paint, and the garbage bag with the beneficial bacteria is obtained. After the sticky paint is formed by mixing the beneficial bacterium base material and the sticky matter free of germicidal ingredients and liable to absorb water, the inner wall of the garbage bag is coated with the sticky paint, so that kitchen garbage ferments in a beneficial bacteria fermentation manner under promotion of the beneficial bacteria at first time when loaded into the garbage bag, the garbage is prevented from going bad and decaying in the long-time transportation process from classified collection to transferring, pollution to the environment from odor generated by decaying of the kitchen garbage is reduced, and the rate of later utilization of the kitchen garbage is increased.