The invention relates to food processing and brewing, in particular to a production method of sea buckthorn fruit wine. The method comprises the following steps: using fresh frozen sea buckthorn fruits as raw materials, selecting undamaged fresh frozen sea buckthorn fruits, cleaning and then preheating at the temperature of 40 to 45 DEG C to be unfrozen, filtering to remove pomace by a 20-mesh sieve after mechanically breaking, then carrying out centrifugal separation to remove puree so as to obtain sea buckthorn juice; then carrying out pasteurization, deacidification, enzymolysis and separation of fruit oil, preparing pulp, adjusting sugar, performing primary fermentation and post-fermentation, filtering, carrying out clarification, decolorization and storage, blending and filling to obtain a finished product. The fruit wine disclosed by the invention is bright in color and luster, keeps nutritional values, aromas and taste of the original fruits and has high juice yield.