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发明公开
CN106244667A 用于快速鉴别禽多杀性巴氏杆菌致病力的培养基及其应用 有权

用于快速鉴别禽多杀性巴氏杆菌致病力的培养基及其应用
摘要：
本发明公开了一种用于快速鉴别禽多杀性巴氏杆菌致病力的培养基，以及利用该培养基鉴别禽多杀性巴氏杆菌致病力的方法，所述培养基包括脱水小牛脑浸粉、胰蛋白胨、甘油、柠檬酸钠、硫酸锰、硫酸镁、硫酸锌、磷酸氢二钾、卡拉胶、水解乳蛋白、葡萄糖、L-谷氨酸钠、维生素B6、维生素B12、马血清、鸡红细胞裂解液、纯化琼脂粉和去离子水。所述方法可以直观的进行禽多杀性巴氏杆菌菌种筛选，具有简易、方便、快速等优点。与传统的动物致死性试验相比，减少了实验动物的用量，改善了动物福利，缩短了实验周期。
摘要（英）：

The present invention discloses a culture medium for rapidly identifying the virulence of avian Pasteurella multocida, and a method for identifying the virulence of avian Pasteurella multocida by using the culture medium, wherein the culture medium comprises brain heart infusion broth, tryptone, glycerol, citrate sodium, manganese sulfate, magnesium sulfate, zinc sulfate, dipotassium hydrogen phosphate, carrageenan, hydrolyzed milk protein, glucose, sodium L-glutamate, vitamin B6, vitamin B12, horse serum, chicken red cell lysate, purified agar powder and deionized water. According to the present invention, with the method, the avian Pasteurella multocida screening can be intuitively performed, the method has advantages of simpleness, convenience, rapidness, and the like, and compared with the traditional animal fatal test, the test of the present invention has advantages of experimental animal consumption reducing, animal welfare improving, and experimental cycle shortening.

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