The invention belongs to a method for measuring biogenic amine in tobaccos. The method includes that liquid-liquid extraction-tandem solid-phase extraction are used as pretreatment means, and the seven types of biogenic amine (including putrescine, cadaverine, histamine, tyramine, spermine, spermidine and tryptamine) in the tobaccos are measured by the aid of high-performance liquid chromatography-mass spectrometry/mass spectrometry (HPLC-MS/MS) processes. Compared with the literature, the method has the advantages that the method suitable for measuring the biogenic amine in the tobaccos is created; the created method is simple and reliable and can be applied to detecting the biogenic amine in the tobaccos; samples can be easily, convenient and quickly analyzed by the aid of the method, and the method is low in detection limit, high in sensitivity and good in repeatability and recovery rate.