The invention discloses a biological chip used for detecting human heat shock proteins. The biological chip comprises a glass substrate, and a detection plate arranged on the glass substrate; the lower bottom surface of the detection is pressed against the upper surface of the glass substrate; a detection liquid adding hole and a group of detection holes are arranged on the detection plate; the detection liquid adding hole is connected with each of the detection holes via channels; and each detection hole is provided with a liquid absorption block placing hole used for accommodating liquid absorption blocks, wherein each liquid absorption block placing hole is arranged on the detection plate outside the corresponding detection hole. The biological chip is reasonable in design; and one of the biological chip can be used for detection of a plurality of kinds of human heat shock proteins at the same time, so that the biological chip is saved, detection cost is reduced, and detection efficiency is high.