The invention provides a wire clamping and cutting device in audio wiring operation, which is convenient for audio wire clamping and cutting. A wire cutting assembly is arranged on the rear side of a bracket. The wire cutting assembly mainly comprises a knife holder, blades, a first screw and a first nut. Three long grooves are arranged at the upper part, the middle part and the rear part of the rear side of the bracket. The knife holder is movably arranged in the middle long groove. Two rows of blades which are vertically fixed on the front side of the knife holder. The first nut is fixed on the rear side of the knife holder. The first nut matches the first screw. The first screw is rotationally arranged on bracket. The first nut is slidably arranged on the bracket. A wire clamping assembly is arranged on the rear side of the bracket. The wire clamping assembly mainly comprises a card holder, a connecting plate, a second nut and a second screw. The card holder is movably arranged in upper and lower long grooves. A number of equidistantly spaced cards are fixed on the front of the card holder. Each card is provided with a wire clamping port. According to the invention, audio wire clamping and cutting can be realized in turn.