The invention belongs to the technical field of biological prevention and control for agriculture and forestry pests, and particularly relates to a method for propagation of aphidius gifuensis for multiple times by using tobacco plants. The method comprises the following steps of breeding of myzus persicae host plants, propagation of the aphidius gifuensis for the first time, propagation of the aphidius gifuensis for the second time, propagation of the aphidius gifuensis for the third time, and propagation of the aphidius gifuensis for the fourth time. The method has the advantage that the developed root system of a tobacco plant is sufficiently utilized, and new leaves are promoted to germinate and quickly grow, so that the tobacco plant can be reutilized, the secondary seedling cultivation and transplanting can be avoided, the labor intensity is decreased, the time is saved, and the propagation efficiency of the aphidius gifuensis is greatly improved.