The invention relates to a cisatracurium besilate freeze-dried powder injection and a preparation method thereof, and specifically relates to a stable cisatracurium besilate freeze-dried powder injection and a preparation method thereof. The freeze-dried powder injection prepared by the invention overcomes the defect that the existing cisatracurium injection or freeze-dried powder injection needs to be stored at a low temperature, and has favorable preparation dissolvability and stability, uniform structure and good formation property.