The invention discloses an extraction, purification and detection method for euphausia superba non-phenolic weakly-basic alkaloid. The method comprises the steps that 1, euphausia superba is extracted with methanol and filtered, and filtrate is concentrated to obtain a methanol extract; 2, the methanol extract is extracted with ether and filtered, and ether liquid evaporates to obtain an ether extract; 3, the ether extract makes contact with ether to be dissolved, ether extract liquid is obtained and makes contact with an acid aqueous solution, extraction is conducted, and an acid aqueous phase is obtained; 4, the acid aqueous phase and chloroform are extracted three to four times, and a chloroform layer A is obtained; 5, the chloroform layer A is extracted with a sodium hydroxide aqueous solution, and an alkaline water layer and a chloroform layer B are obtained; 6, a solvent is removed, and the euphausia superba non-phenolic weakly-basic alkaloid is obtained. According to the extraction, purification and detection method for the euphausia superba non-phenolic weakly-basic alkaloid, the extraction technology of the euphausia superba non-phenolic weakly-basic alkaloid is optimized, by accurately determining the frequency of extraction in category separation, the relative content of alkaloid in a crude extract obtained after category separation is conducted directly reaches 80% or above, and dioctyl adipate and oleamide can be easily and efficiently removed.