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发明授权
CN106282414B 用于H5N6禽流感病毒检测的试剂、检测方法及应用 有权

用于H5N6禽流感病毒检测的试剂、检测方法及应用
摘要：
本申请公开了用于H5N6禽流感病毒检测的试剂、检测方法及应用。本申请用于H5N6禽流感病毒检测的试剂，包括引物对和探针，探针在引物对的扩增靶标区域内，引物对上游引物为Seq ID No.1所示序列，引物对下游引物为Seq ID No.2所示序列，探针为Seq ID No.3所示序列或Seq ID No.3所示序列的反向互补序列。本申请用于H5N6禽流感病毒检测的试剂，对禽流感亚种H5N6具有很强特异性，能够准确灵敏的从众多禽流感病毒中特异性的检测出H5N6，为紧急疫情快速诊断和大规模样品的快速检测奠定了基础，特别适合于检验检疫等部门使用，为保障社会安全、避免疫情传播扩散提供了有力的科学工具。
摘要（英）：

The invention discloses a reagent used for H5N6 avian influenza virus detection, a detection method and application. The reagent used for H5N6 avian influenza virus detection comprises a primer pair and a probe, wherein the probe is in the amplification target region of the primer pair; a forward primer of the primer pair is a sequence shown in Seq ID No.1; a reverse primer of the primer pair is a sequence shown in Seq ID No.2; the probe is a sequence shown in Seq ID No.3 or a reverse complementary sequence of the sequence shown in Seq ID No.3. The reagent used for H5N6 avian influenza virus detection has strong specificity toward the avian influenza subspecies H5N6, can specifically detect H5N6 from numerous avian influenza viruses accurately and sensitively, lays the foundation for rapid diagnosis of emergency epidemics and rapid detection of large-scale samples, is especially suitable for inspection and quarantine departments and other departments to use and provides a powerful science tool for guaranteeing social security and avoiding epidemic spreading and diffusion.

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