The invention discloses a method for rapidly detecting mulberry anthocyanins and flavonoids by utilizing UPLC-TUV, detecting conditions for ultra high performance liquid chromatography detection and a sample treating method. According to the method, three types of anthocyanins and five types of flavonoids in mulberries can be rapidly separated and detected at a certain flow velocity and in a proportional gradient elution mode. The method is sensitive, high in separating degree and good in repeatability.