The invention is suitable for the field of 3D display technology, and provides a display method and a display device of a glass-free 3D image, and a liquid crystal display screen. The display method comprises the steps of determining relative positions of a cylindrical lens and subpixels; classifying the subpixels according to the relative positions for obtaining N kinds of subpixels, and filling the N kinds of subpixels into N corresponding parallax pictures. According to the display method, the display device and the liquid crystal display screen, each kind of pixels correspond with one parallax picture. Compared with the prior art, the display method, the display device and the liquid crystal display screen are advantageous in that the number of parallax pictures is greatly increased; and in viewing of a user, smooth parallax jump transition is realized; and furthermore the display method, the display device and the liquid crystal display screen accord witih light field spatial distribution. The display method, the display device and the liquid crystal display screen settle a problem of human eye discomfort or fatigue caused by remarkable parallax jump caused by limited number of parallax pictures in prior art.