The invention discloses a production method of an AGM partition plate capable of preventing an electrolyte from being layered. The production method comprises the following steps of (1) preparing modified bamboo fiber; (2) preparing a material; (3) pulping; (4) forming and making paper; (5) performing one-time drying; (6) rolling; and (7) performing secondary drying. The production method is simple in process step, low in production and high in maneuverability, the fabricated AGM partition plate has larger electrolyte absorption amount and high wettability and electrolyte retention ability, the electrolyte is uniform in distribution, high in porosity, uniform in air gap and excellent in integral performance, the battery performance is favorably improved, and the service lifetime of a battery is favorably prolonged.