The invention discloses a method and a kit for quantitative detection of an effect of treating hepatic failure of glucocorticoid. The kit comprises a reagent for performing bisulfite modification on a genome DNA, five premixed PCR (polymerase chain reaction) reaction systems, a methylation specific primer pair and a Taqman fluorescent probe aiming at a target gene SOCS1 starter, a specific primer pair and a Taqman fluorescent probe aiming at a reference gene ALU-C4, a reagent for extracting a peripheral blood genome DNA, a positive control and a negative control. The whole process from blood collection to quantitative detection of an SOCS1 gene methylation level related to hepatic failure prognosis can be completed by using one kit, and the prognosis and the treatment effect of a hepatic failure patient can be judged by detecting the SOCS1 gene methylation degree in blood. The method and the kit have the advantages of simple and convenient operation, high sensitivity, accurate and reliable result, and good stability.