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发明公开
CN106319071A 用BsmAI鉴定人乳腺癌LINC-ROR基因rs4801078多态性的方法 审中-实审

用BsmAI鉴定人乳腺癌LINC-ROR基因rs4801078多态性的方法
摘要：
本发明公开了一种用BsmAI鉴定人乳腺癌LINC-ROR基因rs4801078多态性的方法，包括以下步骤：抽提样品基因组DNA；提供扩增人LINC-ROR基因多态性rs4801078位点附近序列的正向引物和反向引物，以所述待测人基因组DNA为模板，进行PCR扩增反应，获取扩增产物；使用限制性内切酶对所述扩增产物进行酶切，获取相应的酶切产物；将酶切产物采用3％的琼脂糖凝胶进行电泳，以判定LINC-ROR基因多态性rs4801078位点的各基因型。本发明建立的方法简单、快速、安全、准确、灵敏度高，值得在临床和研究中推广，并可借鉴用于其它多态性基因的分型。
摘要（英）：

The invention discloses a method for identifying human breast cancer LINC-ROR (Long Intergenic Non-Protein Coding RNA, Regulator of Reprogramming) gene rs4801078 polymorphism by BsmAI. The method comprises the following steps of extracting sample genome DNA (deoxyribose nucleic acid); providing forward primers and reverse primers of sequences near LINC-ROR gene polymorphism rs4801078 sites of an amplification person; performing PCR (polymerase chain reaction) by using the human genome DNA to be tested to obtain an amplification product; performing enzyme digestion on the amplification product by using restriction enzymes to obtain corresponding enzyme-digested products; performing electrophoresis on the enzyme-digested products by 3-percent agarose gel for judging each genotype of the LINC-ROR gene rs4801078 polymorphism sites. The built method is simple, fast, safe and accurate; the sensitivity is high; the method is worthy of being popularized in clinics and research, and can be used for other polymorphism gene distribution.

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