The invention discloses an inverted ventilation test method for a multi-stage fan station ventilation system in an underground metal mine. The adopted multi-stage fan station ventilation system is composed of a downcast shaft (1), a return shaft (7), an upper middle segment horizontal roadway, a lower middle segment horizontal roadway, a middle segment air intake fan (3), an upper middle segment horizontal air door (8), a lower middle segment return fan (5'), a lower middle segment air intake fan (3'), a lower middle segment horizontal air door (8'), a lower middle segment return air raise (9') and an upper middle segment return air raise (9). The multi-stage fan station ventilation system is divided into an air intake side and an air return side according to the demarcation point of the positive relative static pressure and the negative relative static pressure of an air path, and what kind of inverted ventilation mode is selected according to the location where a catastrophe occurs. According to the method, a basis is provided for inverted ventilation mode selection of an inverted ventilation test of the multi-stage fan station ventilation system, the inverted ventilation process is easy to operate, whole-mine inverted ventilation can be quickly achieved, and the quick responsiveness of mine inverted ventilation serving as an emergency rescue measure of the mine is fully exerted.