The invention discloses an application interface implementation method based on a railway WebGIS and an application system. Relevant applications of railway are implemented by combining a geographical information database and a railway business database on the basis of the railway WebGIS. The method mainly comprises the following steps: carrying out relevant operations of railway kilometer marks by accurately positioning on a railway line in a block storing and searching manner, and inquiring according to conditions on the railway line and marking points; alarming railway areas by carrying out high-brightness alarm and display for accident areas of the railway, warning workers and rapidly inquiring material conditions around accidents; positioning and displaying devices along the railway line by rapidly accessing to the devices as well as positioning, displaying and viewing device positions; measuring distance on the railway line by accurately calculating traveling distance between any two points on the railway line and completely planning the path of the railway. Through the application interface implementation method based on railway WebGIS and the application system, the complete functions of the system are ensured; the data accessing efficiency and the positioning accuracy of the devices along the railway are improved.