The embodiment of the invention provides a high-frequency and high-voltage power supply, and belongs to the field of power supply. The high-frequency and high-voltage power supply comprises a filtering circuit, two high-frequency inversion generation main circuits, a high-frequency step-up transformer, a single-phase rectifier bridge, a controller module, two rectification driving circuits, two inversion driving circuits, a wire-inlet current transformer, a wire-inlet conditioning circuit, and a secondary voltage current acquisition conditioning circuit. By employing the mode of series connection of two high-frequency inversion generation main circuits, the input voltage of the high-frequency step-up transformer is increased, and the leakage inductance of the high-frequency step-up transformer is reduced in the condition of a certain output voltage; a three-phase fully-controlled rectifier bridge is employed in each high-frequency inversion generation main circuit, respective output bus voltage can be flexibly adjusted in real time, and the wire-inlet voltage of the high-frequency step-up transformer is adjusted; and finally, the power supply can simultaneously consider adjustment of voltage and frequency, and the switching frequency of the power supply is increased.