The invention discloses a natural Qiang medicine spray which comprises, by weight parts, 5-20 parts of ruma volatile oil, 1-5 parts of mint volatile oil, 1-5 parts of perilla volatile oil, 1-5 parts of zunguba volatile oil and 1-10 parts of benzalkonium bromide. A method for preparing the natural Qiang medicine spray comprises the steps of weighing each component according to a formula, adding deionized water with the weight being 80-100 times that of the mixture, placing the mixture in a mixer to perform mixing evenly, performing filtration through a filter, and obtaining filtrate, namely the natural Chinese herbal medicine spray. The preparation method is simple, natural Qiang medicine is taken as a raw material, the cost is low, and the natural Qiang medicine spray is safe and non-toxic and has no stimulation, various crissal pathogenic microorganisms can be effectively killed, and the natural Qiang medicine spray has the advantages of having broad sterilization spectrum and being high in sterilization capability, quick in action speed and good in stability and the like. The natural Qiang medicine spray has no pungent smell after being used, the human body has no discomfort, and the natural Qiang medicine spray does not have side effects.